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IGCSE Biology · 0610

IGCSE Biology: Movement Into and Out of Cells Practice Questions

Diffusion, osmosis and active transport are among the most tested ideas in IGCSE Biology, and the mark is usually lost on the exact wording. These practice questions show you the phrasing examiners want, with worked answers and the common traps.

What you need to know

  • Diffusion is the net movement of particles from a higher to a lower concentration, down a concentration gradient, and is passive.
  • Osmosis is the net movement of water molecules from a higher to a lower water potential through a partially permeable membrane.
  • Active transport moves substances against the concentration gradient and needs energy from respiration, using carrier proteins.
  • A larger surface area to volume ratio, a steeper gradient and a shorter distance all speed up the rate of movement.
High water potential Low water potential partially permeable membrane water
Osmosis: water moves across the partially permeable membrane towards the lower water potential.

Practice questions with answers

Question 13 marks

Define osmosis.

Osmosis is the net movement of water molecules from a region of higher water potential (a dilute solution) to a region of lower water potential (a concentrated solution), through a partially permeable membrane.

Mark schemeWater molecules (1). Higher to lower water potential, or dilute to concentrated (1). Through a partially permeable membrane (1).

Common mistakeWriting that solute or particles move. Osmosis is the movement of water only.

Exam tipAlways name the barrier as a partially permeable membrane. Leaving it out costs a guaranteed mark.

Question 24 marks

A piece of potato is placed in a concentrated salt solution. After one hour it is softer and has lost mass. Explain why.

The salt solution has a lower water potential than the potato cells. Water moves out of the cells by osmosis across the partially permeable cell membrane. The cells lose water and become flaccid, so the potato is softer and its mass decreases.

Mark schemeSolution has lower water potential than cells (1). Water leaves cells by osmosis (1). Named membrane, partially permeable (1). Cells flaccid, so mass and firmness fall (1).

Common mistakeReversing the direction of water movement, or saying the salt enters the potato.

Exam tipLink the direction of water movement directly to the change in mass. State cause then effect.

Question 33 marks

State two differences between diffusion and active transport.

Diffusion is passive and moves particles down a concentration gradient, needing no energy. Active transport moves substances against the concentration gradient and requires energy from respiration, using carrier proteins in the membrane.

Mark schemeDiffusion down gradient or passive (1). Active transport against gradient (1). Active transport needs energy from respiration, or uses carrier proteins (1).

Common mistakeSaying active transport needs energy but not stating that the energy comes from respiration.

Exam tipThe phrase energy from respiration is worth a mark on its own. Write it in full.

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Frequently asked questions

What is the difference between osmosis and diffusion?

Diffusion is the movement of any particles from a high to a low concentration. Osmosis is specifically the movement of water molecules across a partially permeable membrane from a higher to a lower water potential.

Is osmosis active or passive?

Osmosis is passive. It does not need energy from respiration because water moves down a water potential gradient on its own.

Why does active transport need energy?

Active transport moves substances against the concentration gradient, from low to high concentration. Moving against the gradient requires energy, which is released by respiration.

What is water potential in simple terms?

Water potential is a measure of how freely water can move. Pure water has the highest water potential. Adding solute lowers it, so water moves from higher to lower water potential.

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